Part:BBa_I766007:Design

YopH, Rev. MAPK Tyr Phosphatase

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 468
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 468
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 468
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 973
  

Design Notes

Not a standard BioBrick. Cloned using primers containing adaptor regions for combinatorial cloning using type IIs restriction enzyme.

Source

Yersinia Pestis

References

Reference: Protein tyrosine phosphatase activity of an essential virulence determinant in Yersinia KL Guan and JE Dixon PMID: 2166336 Yersinia is the genus of bacteria that is the causative agent in plague or the black death, and on several occasions this organism has killed a significant portion of the world's population. An essential virulence determinant of Yersinia was shown to be a protein tyrosine phosphatase. The recombinant 50-kilodalton Yersinia phosphatase had a specificity for removal of phosphate from Tyr-containing as opposed to Ser/Thr-containing phosphopeptides and proteins. Site-directed mutagenesis was used to show that the Yersinia phosphatase possesses an essential Cys residue required for catalysis. Amino acids surrounding an essential Cys residue are highly conserved, as are other amino acids in the Yersinia and mammalian protein tyrosine phosphatases, suggesting that they use a common catalytic mechanism